Spatial Omics: High-Dimensional CyTOF & IMC Center

As the next-generation single-cell, high-multiplex technology, this platform has become an essential research tool for cell therapy development and immunotherapy clinical trials. To date, this technology has been featured in more than 2,000 high-impact scientific publications worldwide.

Versatile Biomedical Applications

Our CyTOF platform supports a wide range of research and development areas, including:

• Tumor Immunology & Cancer Microenvironment
• Translational Medicine & Biomarker Discovery
• Immunology & Infectious Diseases
• Stem Cell & Developmental Biology
• Neuroscience & Neuroimmunology
• Systems Biology & Multi-omics Integration
• Quality Control & Heterogeneity Profiling of Cell Therapy Products (e.g., CAR-T/NK)
• Mechanism of Action (MOA) Verification in New Drug Development

How CyTOF Works

CyTOF® (Mass Cytometry) combines fluidics-based cell interrogation with inductively coupled plasma mass spectrometry (ICP-MS). By utilizing highly pure stable rare earth metal-tagged antibodies instead of fluorophores, it detects metal signals via mass spectrometry rather than traditional fluorescence.

Workflow:

1. The cell solution is placed into a nebulizer turning the mixture into an aerosol
2. The aeorsol is passed through a heated spray chamber where it is exposed to a plasma torch, which vaporizes and ionizes the cells
3. The ion cloud is passed through a quadrupole to remove biological elements and isolate the mass tags.
4. The mass tags are then separated by their mass-to-charge ratio in a time-of-flight mass spectrometer.
5. Ion signals are collected per cell, resulting in single-cell measurement at subcellular resolution for downstream analysis.

Core Advantages & Features of CyTOF

High Multiplexing: Simultaneously measures 40-plus markers in a single tube, enabling systematic immune profiling while significantly reducing sample volume requirements.

High Resolution: Conducts multi-antigen, multi-parameter analysis at single-cell resolution using mass spectrometry principles.

High Data Quality: Utilizes metal tags to eliminate the limitations of spectral overlap (compensation) and tissue autofluorescence interference common in traditional fluorescence-based methods.

【Platform Extension】Spatial Omics Research via Hyperion XTi™ Imaging System

Powered by proven Imaging Mass Cytometry™ (IMC™) technology, this multiplexed tissue imaging system simultaneously detects 40-plus protein and RNA markers on a single tissue slide (FFPE or frozen) without autofluorescence interference. Beyond comprehensive cellular mapping, it allows researchers to precisely calculate the physical distances between cells and perform advanced spatial neighborhood analysis.

Preclinical & Clinical Advantages of the TMT Center
TMT integrates CyTOF technology to deliver comprehensive solutions with key strategic advantages :

• • Advancing Cell Therapy Development

By combining our existing proteomics platform with CyTOF technology, we identify critical protein biomarkers in cell therapy products. We establish multi-target analysis and precision quantification systems, setting a benchmark for cell therapy quality assessment.

• • Immunotherapy Preclinical Research & Clinical Trials

Preclinical Research: Precisely identify modulated immune cell subsets during drug development, providing accurate quantitative data to accelerate the understanding of drug mechanisms of action (MOA).

Clinical Trials: Operating under the certified TMT quality system, we deliver high-quality immune monitoring during CAR-T clinical trials. Our cell population profiling and real-time monitoring deliver highly efficient efficacy assessment and precise tracking of cell functional status

• • Exosome CMC Characterization & Assay Development

In collaboration with the instrument manufacturer, TMT is pioneering the development of a specialized exosome detection system using mass cytometry in the Pan-Asia Pacific region, establishing a major breakthrough and a premier collaborative platform in the field.

For more information：https://standardbio.com